Skip to main content
. Author manuscript; available in PMC: 2014 Oct 24.
Published in final edited form as: Cell. 2013 Oct 24;155(3):10.1016/j.cell.2013.09.028. doi: 10.1016/j.cell.2013.09.028

Figure 5. Ascl1, but not Brn2 and Myt1l, acts as a pioneer factor in embryonic fibroblasts.

Figure 5

(A). Average FAIRE-seq signal of uninfected MEFs at Ascl1 and Brn2 target sites in MEFs infected with the BAM factors. For each target site, the signal is displayed +/− 250bp from the peak summit. While Brn2 targets are mostly nucleosome-free, the Ascl1 targets are predominantly nucleosome-bound in MEFs.

(B) Left: Average enrichment of individual histone marks in MEFs based on Brn2 target sites in MEFs after infection with the BAM factors. Right: Heat maps of normalized tag densities representing MEFs chromatin marks at the same Brn2 targets sites. The signal is displayed within an 8 kb window centered around the binding sites.

(C) Left: Average enrichment of individual histone marks in MEFs based on Myt1l target sites in MEFs infected with BAM. Right: Heat maps of tag densities representing indicated histone marks at Myt1l targets sites. The signal is displayed within an 8 kb window centered around the binding sites.

(D) Average FAIRE-seq signal of uninfected MEFs at the genomic sites where Ascl1 (blue) or Brn2 (purple) is bound in NPCs. Dotted line represents the FAIRE-seq signal at random sites.

See also Figure S5.