Desnutrin regulates GSIS by activating PPARδ. A) RT-qPCR of PPARα, δ and γ in islets. B) GTT of mice treated with the PPARδ agonist, GW501516 (left), or fed with PPARα agonist, WY 14643 (right). C) GSIS of isolated islets from GW501516 treated mice. D) GSIS of isolated islets from WY 14643 treated mice. E) PPARδ (left) and PPARα (middle) binding activity in islet nuclear extracts, and PPARδ binding activity from GW501516 treated mice (right). F) RT-qPCR in isolated islets from mice treated with GW501516. Male mice, n=4–6, Data are shown as mean ± SEM; *P<0.05, **P<0.01. G) Schematic diagram of desnutrin-PPARδ-mitochondrial axis. See also Figure S4.