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. 2013 Dec 9;124(1):297–310. doi: 10.1172/JCI66947

Figure 3. CQ inhibits OC formation in vitro.

Figure 3

(A) Typical in vitro OC formation time-course. BM-derived WT OCPs were treated with M-CSF (M) for 2 days and with M-CSF plus RANKL (R) for 4 days. CQ was added on day 2 (B), or day 4 (C). Original magnification, ×4. *P < 0.05. (D) WBs of WT OCPs treated with RANKL with or without CQ (10 μM) for 3 days. (E) WBs of Traf3f/f OCPs infected with MSCV-GFP (GFP) or MSCV-Cre-GFP (Cre-GFP) retroviruses and GFP-FACS sorted or (F) cultured with RANKL with or without CQ (2 μM) for 5 days. (G) WBs of whole cell lysates from WT or TRAF3 L-cKO mouse tissues. (H) WBs of BM cells cultured with RANKL with or without CQ (2 μM) for 5 days. **P < 0.01 vs. PBS.