Skip to main content
. 2013 Dec 9;124(1):99–110. doi: 10.1172/JCI70103

Figure 3. CD4+ T cells of EAE Bcl11bF/F/dLck-iCre mice accumulate in the enlarged mLNs, Peyer patches, and SILP, proliferate normally, and do not cause overt colitis.

Figure 3

(A) Gross anatomy of dLNs and mLNs from the indicated EAE groups on day 12 after EAE induction. Data are representative of 10 pairs. (B) Absolute numbers of CD4+ T cells in the indicated groups and tissues on day 12 after EAE induction. *P ≤ 0.05; n = 10 (mean ± SEM). (C and D) FACS analysis of CD4, and IL-17, CD44 and CD25 within the CD4+ T cells–gated population in Peyer patches (PP) (C) and SILP (D) on day 18 after EAE induction. (E) H&E staining of small intestine sections of the indicated groups on day 18 after EAE induction. Scale bars: 400 μm. Data in CE are representative of 3 pairs of mice. (F) FACS analysis of BrdU incorporation (top) and Ki67 levels (bottom) in the indicated tissues and groups of EAE mice. Data are representative of 3 pairs of mice. (G) Proliferation of Bcl11b-deficient and WT cells evaluated by CFSE dilution. Naive CD4+ T cells of Bcl11bF/F/dLck-iCre/2D2 and Bcl11bF/F/2D2 mice were labeled with CFSE and incubated for 3 days with plate-bound anti-CD3 and soluble anti-CD28, followed by FACS analysis of CFSE dilution. Red lines indicate CFSE levels following 3 days of treatment; black lines indicate unstimulated cells after 3 days; and gray shaded areas indicate CFSE levels on day 0. Data are representative of 3 pairs of mice.

HHS Vulnerability Disclosure