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. 2013 Dec 9;124(1):156–168. doi: 10.1172/JCI70259

Figure 3. Effect of AZD0530 on proliferation and cell cycle profile of D2.0R cells on BME or BME plus COL1.

Figure 3

(A) Representative Ki67 immunofluorescence of D2.0R cells on BME (upper panels) or BME plus COL1 (lower panels) treated with 250 nM AZD0530 or vehicle (scale bar: 25 μm), with (B) quantification. *P = 0.04; **P = 0.0058; ***P ≤ 0.0001. (C) Cell cycle profile of D2.0R on BME (left) or BME plus COL1 (right) treated with 250 nM AZD0530 or vehicle for 72 hours. (D) MTS assay of D2.0R cells treated with 250 nM AZD0530 or vehicle for 14 days or for 4 days, after which cells were washed and supplemented with vehicle for 10 days (dashed line indicates the recovery phase).