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. 2013 Dec 9;124(1):398–412. doi: 10.1172/JCI71180

Figure 2. Aberrant accumulation of 2HG in breast cancer and its relationship with genome-wide DNA methylation in breast tumors.

Figure 2

(A) Relative abundance of 2HG in breast tumors and adjacent noncancerous tissue for all paired samples (n = 65, 33 ER-positive and 32 ER-negative) for the discovery set. (B) 2HG quantitation for ER-negative tumor (n = 60) and adjacent noncancerous tissue (n = 29) in the validation set. P value was determined by paired t test (n = 29). Relative abundance of 2HG in 3 benign and 14 cancerous human breast cell lines is also shown. (C) Normalized genome-wide DNA methylation scores were calculated for breast tumors in the discovery set using Human Methylation 450 BeadChips data. 2HG-high tumors (median and above) had a significantly higher DNA methylation score than 2HG-low tumors (below median), as determined by Welch t test.