Fig. 4.

Temperature effect of wild-type and mutant C-SdCen variants binding to R18-Sfi1 in the absence of calcium as measured by ITC. The ITC measurements show the C-SdCen variants binding to XPC at 30 °C (upper graph) and at 37 °C (lower graph). For each graph, the upper panels show the raw data, and the lower panels show the binding isotherms fitted to a one-site model. Peptide solutions (100 μM) were injected into the protein (10 μM) equilibrated in buffer (50 mM MOPS, 100 mM NaCl, 2 mM EDTA, pH 7.5) at 30 °C or 37 °C in the calorimeter cell.