Table 3.
Statistical analysis of differential gene expression. (a) Two-way MANOVA over all genes testing the effects of first exposure (T1), second exposure (T2) and their interaction (T1 : T2). (b) Planned contrast between homologous (ho) and heterologous (ht) exposure. *p ≤ 0.05, **p ≤ 0.01.
| d.f. | Pillai | approx. F | num d.f. | den d.f. | Pr(>F) | |
|---|---|---|---|---|---|---|
| (a) two-way MANOVA | ||||||
| T1 | 2 | 0.69615 | 0.91528 | 14 | 24 | 0.55601 |
| T2 | 2 | 1.01764 | 1.77586 | 14 | 24 | 0.10461 |
| T1 : T2 | 4 | 1.85618 | 1.73166 | 28 | 56 | 0.04044* |
| residuals | 17 | |||||
| gene | contrast | d.f. | sum sq | mean sq | F-value | Pr(>F) |
| (b) planned contrasts | ||||||
| A12 peroxiredoxin |
ho versus ht | 1 | 4.14 | 4.145 | 2.575 | 0.1202 |
| A4 chitinase |
ho versus ht | 1 | 0.62 | 0.623 | 0.208 | 0.6527 |
| L1N adenosylhomocysteinase |
ho versus ht | 1 | 4.86 | 4.858 | 11.258 | 0.00263** |
| TR2N proPO |
ho versus ht | 1 | 11.84 | 11.839 | 5.030 | 0.0333* |
| TR3 SOD |
ho versus ht | 1 | 10.61 | 10.61 | 4.548 | 0.0422* |
| TR4N complement factor B |
ho versus ht | 1 | 3.41 | 3.409 | 7.304 | 0.0124* |