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. 2013 Nov 27;1(6):590–603. doi: 10.1016/j.stemcr.2013.10.008

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© 2013 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Cell Subsets within CD45⁻ Differentiation Stage

(A) Cell subsets present on day 5 of differentiation from Mesp1-cre/Rosa26-eYfp ES cells. The c-KIT expression and May-Grunwald/Giemsa-stained cytospin images of each cell subset are shown. Gray lines in the c-KIT expression panels represent expression in DN cells, which are shown for comparison. Scale bars: 50 μm.

(B and C) Differentiation potentials of each cell subset. EYFP-labeled DP and CD41SP cells formed hematopoietic cell colonies on OP9 cells (B), whereas AA4SP cells (anti-EYFP [Alexa Fluor 488; green]) predominantly produced endothelial sheets (anti-CD31 [Cy3; red]) on OP9 cells (C); signals for the two markers were colocalized in identical cells. Scale bars, 200 μm (B) and 400 μm (C). Representative results from three separate experiments are shown.

See also Figure S1.