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. 2013 Nov 27;1(6):590–603. doi: 10.1016/j.stemcr.2013.10.008

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© 2013 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Differentiation Properties of CD41SP Cells

(A and B) Sort gates (A) and postsort analysis (B) of CD41SP cells.

(C–F) Differentiation potential of CD41SP cells. CD41SP cells cultured on OP9 cells (C–F and H) or OP9DL1 cells (G) for additional 1 day (C), 2 days (D), 3 days (E), 10 days (H), 2 weeks (G), or 3 weeks in the presence of interleukin-7 (F) were analyzed by flow cytometry (C, D, F, and G), RT-PCR (E), or May-Grunwald/Giemsa staining (H). Small plots shown at bottom right (F and G) and open lines in histograms (C and D) show control staining. The black and white arrowheads in (H) indicate macrophages and neutrophils, respectively. Scale bar, 100 μm. Representative results from at least two separate experiments are shown.

See also Figures S2 and S3.