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. 2013 Nov 27;1(6):590–603. doi: 10.1016/j.stemcr.2013.10.008

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© 2013 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Differentiation Kinetics of CD41SP Cells

(A and B) Sort gates (A) and postsort analysis (B) of CD41SP cells used for the experiments shown below.

(C) The time course of CD41SP cell differentiation. Lower panels show the expression pattern within the CD45⁺TER119⁻ cell fraction. Control staining using day 10 cells is shown on the right.

(D) Comparison of day 6 and day 10 CD45⁻TER119⁺ cells. TER119 expression level and cell size (FSC) (left) and representative May-Grunwald/Giemsa-stained cytospin images of sorted CD45⁻TER119⁺ cells (right) are shown. Scale bars, 25 μm.

(E) Real-time quantitative PCR for β-globin expression on day 6 and day 10 cells. Gene expression levels are shown after internal normalization with Hprt and shown as expression relative to day 6 cells (value = 1). Values are expressed as mean ± SD. Representative results from at least two separate experiments are shown.

See also Figure S4.