Figure 2.

A Single TF Induces Myocytes from ESCs

(A) Myod1, Mef2c, Tcf3, and Esx1 are identified, whose expression shifted the transcriptome of ESCs toward skeletal muscle within the correlation matrix.

(B and C) Immunostaining for MHC or MYOGENIN at differentiation for 5 days used ESCs carrying Myod1, Mef2c, Tcf3, or Esx1 gene. Upper panels show Dox+. Lower panels present Dox−. Scale bars, 200 μm.

(D) Quantitative evaluation of MHC⁺/MYOGENIN⁺ cell percentages on day 7 of differentiation used ESCs carrying Myod1, Mef2c, Tcf3, or Esx1 gene (three independent experiments, SEM). ND, not detected.

(E) Analysis of cell fusion between myocytes derived from ESCs and C2C12 cells is shown. White arrows indicate the fusion of myocytes and C2C12 cells. Scale bars, 100 μm.

See also Figures S1 and S2.