Figure 7.

Synthetic mRNAs Induce Myocytes, Hepatocytes, Blood Cells, or Neurons from ESCs

(A) A schematic representation shows the experimental design for synthetic mRNA transfection to generate target organ cells from ESCs.

(B) Immunostaining for MHC and MYOGENIN at differentiation 11 days with Myod1 mRNA is presented. Scale bars, 200 μm.

(C) Quantitative evaluation of MHC⁺/MYOGENIN⁺ cell percentages on day 11 of differentiation with Myod1 mRNA (three independent experiments, SEM) is shown.

(D) Immunostaining for ALBUMIN and PAS staining on day 11 of differentiation with Hnf4a mRNA is presented. Scale bars, 200 μm.

(E) Amounts of ALBUMIN in the culture media, measured on day 14 of Hnf4a mRNA-transfecting ESC differentiation (three independent experiments, SEM; ^∗p < 0.01 versus control), are shown.

(F and G) FACS analysis for CD45+ cell appearance with Sfpi1 mRNA (three independent experiments, SEM; ^∗p < 0.01 versus control) is presented.

(H and I) Immunostaining for TUJ1, MAP2, SYNAPSIN, TH, ISL1/ISL2, or GABA at differentiation 11 days used Ascl1 mRNA. Scale bars, 200 μm.

(J and K) FACS analysis on day 8 of differentiation for PSA-NCAM⁺ cell appearance with Ascl1 mRNA (three independent experiments, SEM; ^∗p < 0.01 versus control) is shown.

See also Figure S7.