pSTAT5 levels are increased in HD patient monocytes at baseline.

PBMCs were isolated using gradient density centrifugation, stimulated with either IFNγ (for STAT1 activation), IL-6 (for STAT3 activation) or GM-CSF (for STAT5 activation) and stained for CD64 and pSTAT1, 3 or 5. Flow cytometry was used to assess the amount of pSTAT molecules in untreated and treated CD64+ monocytes. Data is shown as mean pSTAT levels normalised to control levels +/- SEM. Two-way student t test was used for statistical analysis.