Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Dec 23;203(6):1043–1061. doi: 10.1083/jcb.201306019

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Shahbazi et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — CLASP2 and p120 are required to maintain the proper dynamics of MTs at cell–cell contacts. (A) CLASP2-deficient and p120-null mKer with their corresponding scramble and p120^f/f controls stained for ECad and α-tubulin. (B) Quantification of the number of MTs that reach an area of cell–cell contact. Only regions with well-formed AJs were selected for the analysis. (n = 60 cells, 2 independent experiments). Data are represented as means ± SEM; *, P < 0.04; ***, P < 0.0005, Student’s t test. (C) CLASP2-deficient and p120-null mKer with their corresponding controls (scramble and p120^f/f) were grown with calcium for 4 h followed by addition of 5 µM nocodazole (noc) for 30 min and extraction of monomeric tubulin. Cells were stained for ECad and α-tubulin. (D) Quantification of the number of resistant MTs present at AJs after nocodazole treatment corresponding to C. Only regions with well-formed AJs were selected for the analysis (n = 30 cells, 2 independent experiments). Data are normalized to control values and represented as means ± SEM; *, P < 0.05; **, P < 0.002, Student’s t test. (E) EB3-GFP was expressed in scramble control cells, CLASP2-deficient cells (CLASP2 knockdown [KD]), p120 control cells (p120^f/f), and p120-null cells (p120^Δ/Δ). Time-lapse images were taken every 2 s. Individual EB3-GFP comets were manually tracked during the last 12 s before reaching an area of cell–cell contact. The speed in each frame is shown. (n = 10 cells; p120^f/f: 58 MTs; p120^Δ/Δ: 58 MTs; scramble: 59 MTs; CLASP2 knockdown: 74 MTs). Data are represented as means ± SEM; ***, P < 0.001; **, P < 0.01; *, P < 0.04, Student’s t test. (F) Frequency distribution of MTs showing aberrant trajectories or straight trajectories upon reaching an area of cell–cell contact from the experiment in E. (G) Individual trajectories of EB3-GFP comets in the xy plane were normalized to the last point of the trajectory (corresponding to the time in which EB3-GFP comets reach AJs and fall off the MT). Circles indicate the mean total distance covered by the p120^f/f and scramble controls. Bars, 25 µm.