Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Dec 23;203(6):1043–1061. doi: 10.1083/jcb.201306019

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Shahbazi et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 8. — Differential distribution of CLASP2 and Nezha in the epidermis. (A) Back skins from WT newborn mice stained for CLASP2/p120 or Nezha/p120. The arrow denotes the basal distribution of CLASP2, and the asterisk indicates suprabasal Nezha localization. epi, epidermis; derm, dermis. (B) Quantification of CLASP2, Nezha, and p120 levels in the epidermis of newborn WT mice. Individual plot profiles were generated across the epidermis, and mean values of fluorescence intensity relative to those expressed at the basement membrane are shown. Data are represented as means ± SEM. (n = 3 mice, 30 plot profiles per mice per staining). a.u., arbitrary unit. (C) Real-time PCR analysis of mKer isolated from the back skin of newborn mice and FACS sorted according to their levels of α6 integrin into two populations: α6^high (basal mKer) and α6^low (suprabasal mKer). CLASP2 levels were analyzed with two different combinations of primers. Keratin 5 (K5) and loricrin were used as markers of basal and suprabasal mKer, respectively. For each primer pair, values were normalized to the transcript levels of the α6^high population. Data are represented as means ± SEM; ***, P < 0.0004; **, P < 0.002; *, P < 0.03, Student’s t test. (D) WT mKer subjected to a 24-h calcium switch in vitro and immunostained for either CLASP2 or Nezha together with p120 and ECad. Insets are magnifications of the boxed regions. (E) Immunoblot analysis of CLASP2 levels in WT mKer subjected to a calcium-switch time course for the time points indicated in hours. LC, low calcium. (F) Quantification of CLASP2 levels observed by immunoblotting in D. n = 3 independent experiments. Data are normalized to LC values and represented as means ± SEM; *, P < 0.008, Student’s t test. Bars: (A and D, main images) 25 µm; (D, insets) 5 µm.