Table 2.
List of primers to generate p120 and CLASP2 constructs or to perform quantitative RT-PCR analyses
| Procedure | Primer pair | Forward (5′ → 3′) | Reverse (5′ → 3′) |
| Cloning into pHFUW-HA tag | p120FL | GAATTCATGGACGACTCAGAGGTGGA | GAATTCTTAAGCGTAGTCTGGGACGTCGTA-TGGGTAAATCTTCTGCATCAAGGGTGC |
| Cloning into pHFUW-HA tag | p120ΔN | GAATTCATGCCCCCACCTTCAAAC | GAATTCTTAAGCGTAGTCTGGGACGTCGTA-TGGGTAAATCTTCTGCATCAAGGGTGC |
| Cloning into pGEX-6P1 | p120FL | ATAAGAATGCGGCCGCGAATTCATG-GACGACTCAGAGGTGGA | ACGCGTCGACCTGCAGCTAAATCTTCTG-CATCAA |
| Cloning into pGEX-6P1 | p120ΔN | ATAAGAATGCGGCCGCGAATTCATG-CCCCCACCTTCAAAC | ACGCGTCGACCTGCAGCTAAATCTTCTG-CATCAAAA |
| Cloning into pGEX-6P1 | p120N | GAATTCATGGACGACTCAGAGGTGGA | CGGTCGACGAATTCCTACATTCCCTTTCGC-AAACTATCCAA |
| Cloning into pGEX-4T1 | CLASP2FL | TCCCCCGGGAATGGAGCCCCGCAGC-ATGGA | TCCGCGGCCGCCTAACTTTGTCCAGAA-ACATCAGT |
| Cloning into pGEX-4T1 | CLASP2ΔN | TCCCCCGGGAGGAAATCCTGCCAA-CAGTGC | TCCGCGGCCGCCTAACTTTGTCCAGAA-ACATCAGT |
| Cloning into pGEX-4T1 | CLASP2ΔC | TCCCCCGGGAATGGAGCCCCGCA-GCATGGA | TCCGCGGCCGCCTAAGAGCGTGGAG-AGGAGTG |
| Cloning into pEGFP-C1 | CLASP2TOG | GCAGGTACCGCCATGGGAAATCCT-GCCAACAGTGCAAGG | GCACCGCGGTTACCATTTGGAAGAAA-AAGGG |
| RT-qPCR | Total CLASP2 | TTGTCGTCCTCTGTCAGTGC | TGCCACGTCTTCTGTCTGTC |
| RT-qPCR | CLASP2-α | AAAGAGACATTCCCCCTGCT | TTGCACTGCTGACAGGATTC |
| RT-qPCR | Keratin 5 | CAGTGTGCCAACCTCCAGAACG | AGCCCGCTACCCAAACCAAGAC |
| RT-qPCR | Loricrin | TCACTCATCTTCCCTGGTGCTT | GTCTTTCCACAACCCACAGGA |
| RT-qPCR | GAPDH | CGTAGACAAAATGGTGAAGGTCGG | AAGCAGTTGGTGGTGCAGGATG |
qPCR, quantitative PCR.