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. 2013 Dec 23;203(6):1021–1041. doi: 10.1083/jcb.201305088

Figure 5.

Figure 5.

SKAP-Hom heterodimerizes with and is functionally redundant with SKAP55. (A) Domain structures of the SKAP55 and SKAP-Hom chimeras. (B) Cells were lysed and Western blotted to determine relative expression of the SKAP55 (WT) and SKAP-Hom (Hom) chimeras (n = 3). (C) J14.SY and JSKAP.SY cells transiently expressing mRFP1 or SKAP-Hom.mRFP1 were stimulated, imaged, and presented as in Fig. 2 B. See Tables 1 and 2 for microcluster properties and experiment numbers. Bars: (MOT and conventional images) 10 µm; (kymographs) 5 µm × 60 s. (D) Composite microcluster traces for the conditions examined in C; numbers in parentheses indicate the total number of cells examined. Line intensity corresponds to the fraction of microclusters surviving; arrowheads identify points of half-maximal microcluster dissociation. (E) Fraction of cells scored as displaying unstable contacts (n = 3). (F and G) Fractional retention on fibronectin-coated or anti-CD3-coated coverslips, as described in Fig. 4 (n = 3). Error bars indicate mean ± SEM. From parental J14.SY cells (with or without mRFP1): *, P < 0.05; **, P < 0.01. From JSKAP.SY (with or without mRFP1): #, P < 0.05; ##, P < 0.01.