Table 1.

Impact of SKAP55 mutants on SLP-76 microcluster dynamics

SKAP55 chimera	Persistence	P	Inward movement	P	Max speed	P	% SLP-76 in clusters	P	Clusters tracked per diameter	P	Totalcells	n (experiments)
	s		µm		nm/s
mRFP1	210.5 ± 7.20		3.0 ± 0.2		147.7 ± 6.1		62.0 ± 2.8		15.1 ± 0.48		46	19
KD	115.8 ± 38.2	**	0.6 ± 0.1	**	57.8 ± 8.6	**	27.3 ± 6.9	**	12.5 ± 1	*, ††	10	3
WT	215.7 ± 7.52		3.4 ± 0.3		150 ± 8.6		72.1 ± 2.3		17.2 ± 0.87	*	29	15
WR	129.3 ± 18.9	**, ††	1.3 ± 0.3	**, ††	127.8 ± 31.9		37.6 ± 6.6	**, ††	13.6 ± 1.8		8	3
SH3	150.5 ± 16.4	**, ††	1.4 ± 0.5	**, ††	104.6 ± 23.4	*, †	41.9 ± 15.4	*, ††	9.6 ± 1.5	**, ††	10	5
ΔPH	222.9 ± 2.1		2.8 ± 0.2		154.2 ± 9.6		77.3 ± 2.9		15.3 ± 0.33		10	3
3YF	169 ± 16.4	*, ††	2.3 ± 0.3		153.5 ± 17.4		66.5 ± 5.1		16.4 ± 0.98		10	4
ΔDM	175.9 ± 23.3		1.5 ± 0.2	**, ††	137.7 ± 15.1		44.9 ± 5.2	**, ††	13.3 ± 1.5	†	10	3
DM	133.1 ± 5.9	**, ††	1.1 ± 0.1	**, ††	101.6 ± 6.2	**, †	32 ± 4.0	**, ††	14 ± 2		10	3
TD	227.2 ± 11.6		2.5 ± 0.2		129.1 ± 23.8		73.1 ± 1.1		14 ± 0.95	†	10	3

SLP-76 microcluster dynamics in J14.SY cells expressing each of the indicated constructs were measured on a per cell basis by manual tracing of microcluster trajectories from SLP-76 kymographs. Values for each experiment were obtained by averaging these per cell values, weighing each cell equally. KD, knockdown. Values displayed in the table are mean ± SEM for the indicated number of experiments. From parental J14.SY cells expressing mRFP1: *, P < 0.05; **, P < 0.01. From J14.SY cells expressing SKAP55.WT.mRFP1: †, P < 0.05; ††, P < 0.01.