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. 2013 Oct 31;1(6):e00125. doi: 10.1002/phy2.125

Figure 7.

Figure 7

Effects of microtubules, microfilaments, and atypical myosins inhibition on TIMP-1 exocytosis. Changes in VP-sensitive decreases in TIMP-1-DsRed fluorescence were determined in LX-2 cells pretreated with either nocodazole (20 μmol/L) for 30–60 min, cytochalasin D (2 μmol/L) for 1–2 h or varying concentrations of blebbistatin (5–100 μmol/L) for 30 min (n = 4–5 for all experiments). (A) Effect of microtubules inhibitor nocodazole. Nocodazole completely inhibited VP-sensitive TIMP-1-DsRed exocytosis at 30–60 min (n = 5 for all groups; P < 0.01). (B) Effect of microfilaments inhibitor cytochalasin D. Cytochalasin D partially inhibited VP-sensitive TIMP-1-DsRed exocytosis at 1 h (*P < 0.01 vs. control; %P = 0.521 vs. control) and completely inhibited VP-sensitive TIMP-1-DsRed exocytosis at 2 h. (C) Effect of atypical myosins inhibitor blebbistatin. Blebbistatin had no effect on VP-sensitive TIMP-1-DsRed exocytosis at 5 μmol/L but blocked TIMP-1-DsRed exocytosis at 50 μmol/L and 100 μmol/L.