Table 1.

qRT-PCR primer sequences and final primer concentrations for target and housekeeping genes

Primer name	Sequence 5′→3′	Primer conc (μmol/L)	Accession no.
HSD11B-1			NM_001009395.1
Forward	GCGCCAGATCCCTGTCTGAT	0.90
Reverse	AGCGGGATACCACCTTCTTT	0.90
HSD11B-2			NM_001009460.1
Forward	GAGACATGCCGTTTCCATGC	0.45
Reverse	TGATGCTGACCTTGACACCC	0.45
NR3C1			NM_001114186.1
Forward	ACTGCCCCAAGTGAAAACAGA	0.90
Reverse	ATGAACAGAAATGGCAGACATTTTATT	0.90
NR3C2			AF349768.1
Forward	ATGACAGCTCCAAACCAAACACGG	0.90
Reverse	AAATCCTGGAAGTACCTTCGCCCA	0.90
NKX2-1			FJ177515
Forward	ACACAAAGACCAAACTGCTGGACG	0.90
Reverse	GCGTGGGAAACCCATTTGAATCAC	0.90
GATA6			DQ126151
Forward	TCTACAGCAAGATGAACGGCCTCA	0.90
Reverse	TAGAGTCCACAGGCATTGCACACA	0.90
SCNN1-A			AF232715.1
Forward	ACGACAAGAACAGCTCCAACCTCT	0.90
Reverse	GCCGCAGATTAAAGCCAGCATCAT	0.90
SCNN1-B			AF065146.1
Forward	AGTGGTTCTGGACCTGTTTGAGGA	0.45
Reverse	CATGTGGTTCCATTGTGGCTGCAT	0.45
SCNN1-G			AF250862.1
Forward	TCGTGCTTCCAGGCAAAGATGGTA	0.45
Reverse	TTAAAGCTGCAGGCTTCCTTGCAC	0.45
ATP1B1			NM_001009796
Forward	TGCCTTTCGTCCTAACGATCCCAA	0.45
Reverse	CTGGGCACATTGCCACAATCTTCA	0.45
AQP1			NM_001009194.1
Forward	AAAGTGTCACTGGCCTTTGGGTTG	0.45
Reverse	ATGTACATGATGGCCCGGAGGATA	0.45
AQP3			AF123316.1
Forward	TCACTTGAACCCTGCTGTGACCTT	0.05
Reverse	ACCCGAAGATAATTCCAGCACCCA	0.05
AQP4			NM_001009279
Forward	TGGGAAATTGGGAGAACCACTGGA	0.45
Reverse	GGCAGCTTTGCTGAAGGCTTCTTT	0.45
AQP5			NM_001009273
Forward	CAATCTGGCTGTCAATGCGCTCAA	0.45
Reverse	AGTCAGTGGAAGAGAAGACGCACA	0.45
SFTP-D			AJ133002.1
Forward	GGCCACAGCCCAGAACAA	0.3
Reverse	AAGTACCCTCCTTCCTGGTATCG	0.3
YWAHZ			AY970970
Forward	TGTAGGAGCCCGTAGGTCATCT	0.45
Reverse	TTCTCTCTGTATTCTCGAGCCATCT	0.45

Accession numbers refer to the published cDNA sequences from which the primer sequences were designed.