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Effects of OXA on insulin secretion in INS-1 cells. Cells were exposed to 10−6 M OXA in the presence or absence of PF-04691502 (AKTi) (10−6 M), wortmannin (PI3Ki) (10−8 M), or SB334867 (OX1Ri) (10−6 M), or a combination of these antagonists. Insulin content was assessed via ELISA. Data are presented as mean ± SEM based on triplicate determinations from a representative experiment. Asterisks indicate significant differences compared to control (**P < 0.01).
