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. 2013 Dec 23;8(12):e83607. doi: 10.1371/journal.pone.0083607

Figure 2. Analytical sensitivity and definition of threshold for mutational call.

Figure 2

A-F) the polymorphic c.2470 G>A substitution was observed with the following dilutions of DNA not carrying the polymorphism: 1:1 (A), 1:2 (B), 1:10 (C), 1:100 (D), but not at 1:1000 (F). At 1:100 dilution the c.2470 G>A substitution was observed when the total number of reads allowed to detect at least 10 reads with the c.2470 G>A change (this means at least 1,000 total reads) (D). The c.2470 G>A substitution was not observed when the total number of reads was not sufficient to detect at least 10 reads with the c.2470 G>A change (E).