Table 1. qPCR Primer Sequences.

Marker	Gene	Primer	NCBI	Forward primer 5′-3′	Reverse primer 5′-3′	Ref
	Symbol	Bank ID	Accession #
GAPDH	GAPDH		NM_002046	ATGGGGAAGGTGAAGGTCG	TAAAAGCAGCCCTGGTGACC	[39]
Axin 2	AXIN2	195927058c2		TACACTCCTTATTGGGCGATCA	AAGTTCGGAACAGGTAAGCAC
β-catenin	CTNNB1			TGCCAT TCCACGACTAGTTCAG	CGTACG GCGCTGGGTATC	[40]
Dickkopf 1Homolog	DKK1		NM_012242.2	GGAAGCGCCGAAAACGCTGC	TCTGGAATACCCATCCAAGGTGCT
Glycogen SynthaseKinase 3 Beta	GSK3B			AACTGCCCGACTAACAACAC	ATTGGTCTGTCCACGGTCTC	[41]
AlkalinePhosphatase	ALPL		NM_000478	GGGAACGAGGTCACCTCCAT	TGGTCACAATGCCCACAGAT	[42]
Runt-RelatedTranscriptionFactor 2	RUNX2		NM_001024630	AGTGATTTAGGGCGCATTCCT	GGAGGGCCGTGGGTTCT	[43]
Collagen Type 1Alpha 1	COL1A1		NM_000088	CCTGCGTGTACCCCACTCA	ACCAGACATGCCTCTTGTCCTT	[42]
Osteocalcin	BGLAP		NM_199173	AGCAAAGGTGCAGCCTTTGT	GCGCCTGGGTCTCTTCACT	[42]
Osteonectin	SPARC		BC008011	CCTGGATCTTCTTTCTCCTTTGC	ATCAGGCAGGGCTTCTTGCT	[42]
Osteopontin	SPP1		BC022844	ACCTGAACGCGCCTTCTG	CATCCAGCTGACTCGTTTCATAA	[42]
Msh homeobox 2	MSX2	84452153c1		ATGGCTTCTCCGTCCAAAGG	TCGTCGGGCGAAAACAAGTC
Distal-lesshomeobox 5	DLX5			GACTTCCAAGCTCCGTTCCA	CTGTAGTAGTCAGAATCGGTAGCTGAA	[44]
Cyclin D1	CCND1		NM_053056	CCCTCGGTGTCCTACTTCAA	AGGAAGCGGTCCAGGTAGTT	[45]