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. 2013 Dec 23;8(12):e82847. doi: 10.1371/journal.pone.0082847

Figure 2. Sal and Pio treatment regulate inflammatory mediator gene expression in total AT differently.

Figure 2

Total epididymal AT samples were obtained from the treatment study mice described in Figure 1 (n = 6–8 mice per group). AT inflammation was determined by assessing immune cell infiltration with H&E staining (A) and by examining the expression levels of inflammatory mediator genes by using real-time RT-PCR. The genes that were tested were macrophage-specific marker genes (B), M1 macrophage marker genes (C), M2 macrophage marker genes (D) and adipokine genes (E). The expression of the housekeeping gene GAPDH was used to normalize gene expression. *p<0.05; **p<0.01; ***p<0.001.