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. 2013 Dec 23;8(12):e83511. doi: 10.1371/journal.pone.0083511

Figure 1. Penicillin G (pG) treatment in vitro does not induce classical persistence of C. trachomatis.

Figure 1

HeLa cells infected by C. trachomatis serovar L2 were treated at 3 hpi with either pG (100 IU/mL) or Ad/EHNA, or left untreated. A- At different times after infection, cells were fixed and stained using an anti-chlamydia antibody (green) and Hoechst (blue). Scale bar: 10 µm. B- pG or Ad/EHNA were removed at 32 hpi from culture medium and culture was continued for 43 h. At 75 hpi, cells were processed for the titration of recovered infectious activity. C- HeLa cells infected by C. trachomatis serovar L2 were treated at 3 hpi either with 1, 10 or 100 IU/mL pG or left untreated. In some samples (+), pG was removed at 24 hpi from culture medium and culture was continued. At 48 hpi or 100 hpi, cell layers (left panel) and supernatants (right panel) were processed for the titration of recovered infectious activity. All experiments have been repeated at least 3 times. * correspond to p< 0.05, ** correspond to p <0.01, *** correspond to p < 0.001. See also Figure S1