Figure 3. pG restores apoptotic pathways in the host cells and inhibits RNA biosynthesis of Chlamydia.

A- HeLa cells were infected by C. trachomatis serovar L2 and treated with pG at 2 hpi or left untreated. At 21 hpi, STS was added to the culture medium. Cells were fixed at 48 hpi and stained as in Figure 1. Scale bar: 10 μm. Left panel: data representation from the pictures after calculation of the percentages of apoptotic infected cells. Right panel: Infected-HeLa cells were treated with pG at either 2 hpi or 29 hpi or left untreated. Then, STS was added at 31 hpi. Cells were fixed at 39 hpi and percentages of apoptotic infected cells were determined and presented as in the left panel. B- HeLa cells were infected by C. trachomatis serovar L2 and treated at 1 hpi with either pG or Ad/EHNA, or left untreated. In some experimental conditions (R), pG or Ad/EHNA were removed from culture medium at 32 hpi and the culture was continued for 43 h. At 75 hpi, cells were processed to measure pre-16S and GAPDH RNA expression. The experiments have been repeated 3 times. * correspond to p< 0.05.