Figure 6. Evaluation of mitochondrial transmembrane electric potential (ΔΨ_m) in L. amazonensis promastigotes using the JC-1 fluorochrome.

(A) Values of ΔΨ_m were evaluated over 36 min, before the addition of 2 µM FCCP to abolish the mitochondrial potential. Two concentrations of POSA and ITZ were used (1 and 5 µM) for 48 h of treatment. The ΔΨ_m values are expressed as the ratio of the reading at 590 nm (aggregate) to the reading at 530 nm (monomer). (B) Analysis of ΔΨ_m at the last minute before the addition of 2 µM FCCP. The data suggest that similar alterations in ΔΨ_m are induced by POSA, ITZ, and FCCP. The experiments were performed three times, each time in triplicate, and the figures shown are representative of these experiments. **p<0.05; ***p<0.0001.