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. 2013 Dec 23;8(12):e84766. doi: 10.1371/journal.pone.0084766

Figure 3. Representative SDS PAGE gel picture of.

Figure 3

(A) His-6-PnpA purification by Ni-NTA chromatography, Lane M) Marker, Lane 1) uninduced, 2) induced whole cell lysate, 3) Induced supernatant, 4) pellet, 5) & 6) flow through, 7) wash and lane 8) Elution of His-6-PnpA is showing with a red arrow.(B) His-6-PnpB purification by Ni-NTA chromatography, Lane M) Marker, Lane1) uninduced , 2) induced whole cell lysate, 3) Induced supernatant, 4) pellet, 5) & 6) flow through, 7) wash and lane 8) Elution of His-6-PnpB is showing with a red arrow.