Table 1. ALDH+ and ALDH− subpopulations of breast cancer cells were separated by flow cytometer and cultured in stem cell medium as described in Materials and Methods.
SUM159 | MDA-MB-231 | SKBr3 | ||
250 cells/well | ALDH+ | 2.7±1.5* | 15.7±1.5* | 12.7±1.5* |
ALDH− | 0 | 4.3±1.5 | 4.7±1.5 | |
500 cells/well | ALDH+ | 20±4.9* | 27.7±4.7* | 14.7±1.2* |
ALDH− | 3±2 | 6±6.1 | 7.3±1.5 | |
1000 cells/well | ALDH+ | 27±2.6* | 46±9.5* | 23.3±4* |
ALDH− | 7±2.6 | 27.7±3.1 | 9.3±2.1 |
The numbers of tumosphere generated per 250, 500 or 1000 cells were counted two weeks later. (*p<0.05 vs ALDH−).