Figure 2. Adenoviral Type 5 (Ad5) vector expression in a host organ panel in tumor bearing immunodeficient Rag2^−/−, and hCAR:Rag2^−/− composite mice.

Host organ EGFP reporter expression following intravenous injection of 1.0×10¹¹ viral particles (vp) of either (A, B) Ad5ROBO4-EGFP (ROBO4) or (C, D) Ad5CMV-EGFP (CMV) vectors. A. Ad5ROBO4 vector expression in Rag2^−/− mice is widespread but focal in liver endothelial cells (ECs), and rarely detectable in single splenic microvessels. All other organs are negative for vector reporter expression. B. Multiorgan, EC restricted vascular expression is evident in Ad5ROBO4-injected hCAR:Rag2^−/− composite mice. C. Ad5CMV vector expression in Rag2^−/− mice is detectable in liver hepatocytes, sporadic splenic (inflammatory) and adrenal cells. D. Ad5CMV vector expression in hCAR:Rag2^−/− mice is complex, hepatocyte localized, but decreased in frequency compared to C in liver, mixed inflammatory and endothelial cell localized in spleen, and EC localized in all other organs including brain and skin. A and B: n = 5–6 mice, combined from 3–6 independent experiments, C and D: n = 3–4 mice combined from 3–4 independent experiments Magnification: 100X, Red: endomucin/CD31, Green: EGFP immunofluorescence, Blue: DAPI.