Figure 3. Vascular restricted ROBO4-directed reporter expression in kidney orthotopic and subcutaneous xenograft tumors.

The tumors were harvested from the same Ad vector injected mice whose host organs were depicted in Figure 2. A. Ad5ROBO4 vector expression in Rag2^−/− mice is sporadic yet easily detectable in vascular ECs within tumors in either orthotopic or subcutaneous microenvironments. B. The hCAR transgene markedly increases Ad5ROBO4 vector expression throughout both the orthotopic and subcutaneous tumors following injection into hCAR:Rag2^−/− mice. C. Ad5CMV expression in Rag2^−/− mice is undetectable in either orthotopic or subcutaneous tumors. D. Isolated intratumoral endothelial cell reporter expression in hCAR:Rag2^−/− mice. White line: kidney-tumor boundary; Arrows: glomerular tufts. Magnifications: 40X, first and fourth columns, and 200X, second and fifth columns, 400X, third and sixth columns. Red: endomucin/CD31, Green: EGFP immunofluorescence, Blue: DAPI.