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. 2013 Dec 23;8(12):e83933. doi: 10.1371/journal.pone.0083933

Figure 4. Semiquantitative immunoblotting reveals differential Ad5ROBO4 reporter expression in tumor compared to liver.

Figure 4

A. Immunoblot of EGFP, VE-cadherin, and β-tubulin loading controls in tissue protein extracts from hCAR:Rag2−/− mice injected with either Ad5ROBO4, left three lanes, or Ad5CMV, right three lanes. B. and C. Densitometry analysis of Ad5ROBO4 vector EGFP expression normalized to either VE-cadherin or β-tubulin. D. Densitometry of AdCMV vector EGFP expression. As AdCMV expression was hepatocyte specific, this blot was only normalized to β-tubulin. A–D: Representative immunoblots from n = 4 mice injected with either Ad5ROBO 4 or Ad5CMV vectors. Li: liver, KO: kidney orthotopic tumor, SC: subcutaneous tumor.