Figure 5. Warfarin pretreatment “detargets” the liver without producing multiorgan Ad5ROBO4 vector expression.

A. Ad5ROBO4 injection into vehicle-treated Rag2^−/− mice produced vascular EC expression predominantly in liver and spleen. B. Warfarin pretreatment, 5 mg/kg, on day −3 and −1 prior to Ad5ROBO4 injection vector injection increased the frequency of splenic and lung EC expression and produced sporadic, infrequent expression in kidney and heart. Liver expression was present but diminished. C. AdCMV injection into vehicle treated mice predominantly produced hepatocyte expression with focal RES cell splenic and scattered lung expression. D. Warfarin pretreatment prior to AdCMV injection markedly decreased the frequency of hepatocyte EGFP expression, while increasing sporadic splenic, and lung expression, inducing focal adrenal cellular and rare muscle and skin EC expression. A, B and S5: Representative images from n = 5 mice from 2 independent experiments, C and D: n = 3 mice from 3 independent experiments. 1×10¹¹ vp were injected in each group. Magnification 100X; Red: endomucin/CD31, Green: EGFP immunofluorescence, Blue: DAPI.