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. 2013 Dec 23;8(12):e83933. doi: 10.1371/journal.pone.0083933

Figure 6. Warfarin liver detargeting enhances tumor neovascular endothelial cell reporter expression of the Ad5ROBO4 vector.

Figure 6

A. Ad5ROBO4 produced easily detectable scattered tumor endothelial cell EGFP immunofluorescence in both kidney orthotopic and subcutaneous 786-O tumors in vehicle-treated Rag2−/− mice. B. Warfarin pretreatment markedly enhanced the multiplicity of tumor endothelial cell reporter gene expression within both orthotopic and subcutaneous tumors in Ad5ROBO-injected mice. C. Ad5CMV injection failed to produce detectable tumor EC expression in vehicle-treated, or D. warfarin-treated mice. A–D: tumors from the same mice as in Figure 5. Magnifications: 40X first and fourth columns, 100X second and fifth columns, and 400X third and sixth columns. Red: endomucin/CD31, Green: EGFP immunofluorescence, Blue: DAPI.