Figure 2. Anti-Orai1 antibody binds to native Orai1 on primary immune cells.

A) Representative histogram demonstrating αOrai1 or isotype control binding to primary immune cells (CD3⁺CD4⁺, CD3⁺CD8⁺, and CD19⁺) from the peripheral blood of a healthy individual using 12.5 µg/mL antibody. B) Median Fluorescence Intensity (MFI) of αOrai1 and mIgG1 control binding to memory (IgD⁻CD27⁺) and naïve (IgD⁺CD27⁻) CD19⁺ B cells; antibody used at 12.5 µg/mL. C) Binding of αOrai1 and mIgG1 control to naïve (CD45RA⁺) and memory (CD45RO⁺) CD3⁺CD4⁺ and CD3⁺CD8⁺ T cells. D) Binding of aOrai1 to non-lymphocyte NK cells (CD3⁻CD19⁻CD14⁻CD56⁺), CD86⁺ and CD86⁻ dendritic cells (CD3⁻CD19⁻CD14⁻CD11c⁺HLA-DR⁺), CD14⁺ monocytes, and granulocytes. All data is representative of at least three independent donors.