Table 6. Validation data for determination of fat- and water-soluble vitamins (n = 3).
| Analyte | RT (min)a | RT RSD (%)b | Regression equation | R2 c | LOD (ppb)d | LOQ (ppb)e |
| Fat-soluble vitamins | ||||||
| Retinol (A) | 2.9387±0.0091 | 0.3088 | y = 40.0574x + 339.2849 | 0.9991 | 0.18 | 0.61 |
| Ergocalciferol (D2) | 8.6523±0.0225 | 0.2598 | y = 0.0923x + 11.8327 | 0.9980 | 47.82 | 159.41 |
| D-α-Tocopherol (E) | 9.6253±0.0131 | 0.1356 | y = 41.8311x − 139.1714 | 0.9996 | 0.18 | 0.59 |
| Phylloquinone (K1) | 11.7927±0.0220 | 0.1862 | y = 14.0609x − 412.1498 | 0.9911 | 0.41 | 1.37 |
| Water-soluble vitamins | ||||||
| Thiamine (B1) | 1.1310±0.0017 | 0.1531 | y = 201.1694x − 429.8190 | 0.9969 | 0.04 | 0.13 |
| Riboflavin (B2) | 8.7147±0.0035 | 0.0403 | y = 8.2510x + 175.5517 | 0.9861 | 0.86 | 2.87 |
| Nicotinic acid (B3) | 1.3417±0.0067 | 0.4963 | y = 7.4249x + 57.4607 | 0.9678 | 0.86 | 2.88 |
| Nicotinamide (B3) | 1.6220±0.0017 | 0.1068 | y = 51.5908x + 315.5337 | 0.9775 | 0.14 | 0.46 |
| D-Pantothenic acid (B5) | 2.9730±0.0276 | 0.9291 | y = 1.9568x + 5.3674 | 0.9948 | 2.85 | 9.51 |
| Pyridoxine (B6) | 1.3747±0.0023 | 0.1680 | y = 71.7554x + 399.2698 | 0.9906 | 0.08 | 0.27 |
| D-Biotin (B7) | 7.7440±0.0060 | 0.0775 | y = 30.9773x + 73.1416 | 0.9883 | 0.20 | 0.66 |
| Folic acid (B9) | 7.3167±0.0225 | 0.3075 | y = 1.6740x + 6.3379 | 0.9994 | 3.30 | 11.00 |
| Cyanocobalamin (B12) | 7.7023±0.0168 | 0.2178 | y = 3.6046x + 22.2344 | 0.9733 | 1.27 | 4.22 |
a Retention time (RT) in min.
b Relative standard deviation (RSD).
c Regression coefficients (R2).
d Limits of detection (LOD, 3.S/N).
e Limits of quantitation (LOQ, 10.S/N).