Table 8. Content, type of amino acid, retention time (RT), peak area as quantitated by RP-HPLC.
Amino acid | Abbreviation | Type | RT (min) | Peak area | Content (µg/g) |
L-Arginine | Arg | Non essential | 4.033 | 840667 | 933.667±10.066 |
L-Aspartic Acid | Asp | Non essential | 4.492 | 1477469 | 1635.667±9.609 |
L-Glutamic Acid | Glu | Non essential | 6.325 | 356571 | 355.667±11.015 |
L-Serine | Ser | Non essential | 8.508 | 1352648 | 948.667±7.371 |
Glycine | Gly | Non essential | 9.258 | 2067729 | 1046.333±11.015 |
L-Histidine | His | Essential | 10.467 | 875468 | 1052.667±12.423 |
L-Threonine | Thr | Essential | 12.108 | 1177030 | 958.333±13.650 |
L-Alanine | Ala | Non essential | 12.933 | 1576317 | 1128.333±15.503 |
L-Proline | Pro | Non essential | 13.458 | 1549047 | 1448.333±10.017 |
L-2-amino-n-butyric acid | Abu | Non essential | ND | ND | ND |
L-Valine | Val | Essential | 21.242 | 83423 | 76.333±7.638 |
L-Methionine | Met | Essential | 24.667 | 639545 | 9485.667±10.017 |
L-Cystine HCl | Cys HCl | Non essential | 26.400 | 1573488 | 1262.667±11.719 |
L-Cystine | Cys | Non essential | 27.517 | 419992 | 369.667±11.930 |
L-Isoleucine | Ile | Essential | 29.100 | 116971 | 133.333±10.599 |
L-Leucine | Leu | Essential | 29.850 | 1218013 | 1051.667±10.693 |
L-Nor Leucine | Nor Leu | Non essential | 30.175 | 1929186 | 1265.333±8.737 |
L-Tryptophan | Trp | Essential | ND | ND | ND |
L-Phenylalanine | Phe | Essential | 31.767 | 1006590 | 1157.333±9.504 |
L-Ornithine | Orn | Non essential | 32.683 | 236800 | 115.333±18.771 |
L-Lysine | Lys | Essential | 33.567 | 2260476 | 1334.333±9.609 |
ND: Not detectable.