Table 1. Primer pairs used for amplification of the full-length (CP), the N-terminus truncated (CPΔN) and the C-terminus truncated (CPΔC) fragments of ORF2 of mink astrovirus strains DK5790 and DK7627.

ORF2 geneFragment	Primerdesignation	Sequence (5′ to 3′)	Position in genomeof AY179509	ORF2 product size (bp)
CP of strains DK5790and DK7627	SMC1FSMC2R	TAC TCT TCA ATG GCG TCC GCC AAT CAG TAC TCT TCG AAG GTT CTT TGA GGA AAT TGC	4175–41926499–6482	23252334
CPΔN of strain DK5790	SMC3FSMC2R	TAC TCT TCG ATG TCA TTG AAT TTG ACA TAC TCT TCG AAG GTT CTT TGA GGA AAT TGC	4655–46726499–6482	1842
CPΔC of strain DK5790	SMC1FSMC4R	TAC TCT TCA ATG GCG TCC GCC AAT CAG TAC TCT TCT AAG CAT AAA GGT GCC ACT ACG	4175–41926037–6020	1863
CPΔN of strain DK7627	SMC3FNSMC2R	TAC TCT TCG ATG ATC TCC CTT AAC CTC TAC TCT TCG AAG GTT CTT TGA GGA AAT TGC	NA6499–6482	1854
CPΔC of strain DK7627	SMC1FSMC4RN	TAC TCT TCA ATG GCG TCC GCC AAT CAG TAC TCT TCT AAG GTT GTA GAC AAG CAA GTA	4175–4192NA	1890

F indicates the forward and R the reverse primer. The recognition site for Eam1104I is in italics. The AAG codon for continuing translation of the protein as fusion to the c-Myc tag is underlined (in SMC2R, SMC4R and SMC4RN). NA – not applicable.