Figure 2. Coating and blocking buffer selection.

A. Different coating buffers (Optibind A-L) were tested for maximum surface binding of 11E5, 2D6, and 5E7 (n = 3). B. Blocking buffers (2% BSA, 3% skim milk, 1% casein, and Optiblock solution) were tested for Ac, WSMoV, and MYSV detection (n = 3). An ideal blocking buffer resulted in highest S/N ratios. Error bars indicate ± standard deviations.