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. 2013 Dec 23;8(12):e83336. doi: 10.1371/journal.pone.0083336

Table 5. Identification of genes involved in saponin biosynthesis along with their RPKM values.

Gene Name EC number Transcript ID Total ESTs RPKM value Number of reads
acetyl Co-acetyl transferase 2.3.1.9 contig 662605, 789282, 807132, 807650, 810900 5 420.22 2162
HMG-CoA synthase 2.3.3.10 contig 617027, 642120, 642122, 642652, 643560, 648291, 662091, 685089, 714060, 719432, 730642, 750334, 752180, 787594, 791328 15 588.8 399
HMG-CoA reductase 1.1.1.34 contig 616693, 642352, 728682, 731828, 739034, 750024, 751864, 757982, 761916, 765000, 777172, 789074, 805890 13 684 1144
mevalonate kinase 2.7.1.36 contig 780100, 790236, 808328 3 116.9 337
phosphomevalonate kinase 2.7.4.2 contig 796674, 802836 2 83.9 351
mevalonate diphosphate decarboxylase 4.1.1.33 contig 731872, 807394 2 183.9 932
1-deoxy-D-xylulose-5-phosphate synthase 2.2.1.7 contig 630630, 639174, 659061, 672215, 692780, 701402, 716282, 758550, 770832, 776522, 785368, 788176, 796714, 799960, 801760, 807536, 809736, 812228 18 33.8 31
1-deoxy-D-xylulose-5-phosphate reductoisomerase 1.1.1.267 contig 767146, 807128, 811584 3 49.4 325
2-C-methyl-D-erythritol 4-phosphate cytidylyl transferase 2.7.7.60 contig 697584, 812958 2 35.05 262
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase 2.7.1.148 contig 815196 1 30.19 302
2-C-methyl-D-erythritol, 2,4-cyclodiphosphate synthase 4.6.1.12 contig 809428 1 35.16 200
4-hydroxy-3-methylbut-2-enyl diphosphate synthase 1.17.7.1 contig 768924, 813016, 815362 3 91.9 179
4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1.17.1.2 contig 663803, 674257, 674259, 697268, 713070, 757110, 770076, 775264, 776012, 776014, 780586, 780710, 813560 13 91.6 217
isopentenyl diphosphate isomerase 5.3.3.2 contig 627116, 640576, 677655, 684005, 771148, 793800 6 239.49 128
geranylgeranyl diphosphate synthase 2.5.1.29 contig 669873, 709788, 771708, 791512, 804002 5 37.97 166
farnesyl diphosphate synthase 2.5.1.10 contig 622530, 663119, 669873, 694410, 709520, 709788, 759242, 764802, 771708, 774914, 782044, 785872, 791512, 804002 14 337.61 174
squalene synthase 2.5.1.21 contig 660543, 669241, 676689, 684225, 720378, 762052, 771110, 771878, 776032 9 178.12 119
squalene monooxygenase 1.14.13.132 contig 625956, 650031, 684205, 699388, 718888, 724460, 735738, 749834, 752160, 753330, 759096, 772472, 773000, 788034 14 789.79 897
cycloartenol synthase 5.4.99.8 contig 634716, 641806, 644535, 660567, 679937, 737170, 761218, 762950, 777784, 785022, 796606, 802302, 805366, 814320, 815892 16 177.58 100
β-amyrin synthase 5.4.99.39 contig 664097 1 30.56 21
dammarenediol II synthase 4.2.1.125 contig 663453 1 125.43 85