Figure 4. SPR-derived binding curves of 20 S editosomes to U-insertion (left) and U-deletion (right) pre-mRNA/gRNA hybrid RNAs.

(A) Binding at dilute solvent conditions (Φ/Φ* = 0). (B) Binding at semidilute conditions (Φ/Φ* = 0.9) and (C) at crowded cosolute conditions (Φ/Φ* = 3.3). Inserts: Plots of k_on(obs) = f(conc_20S) for the calculation of k_ass and k_diss. Error bars are relative errors in percent.