Table 3. Statistically important metabolites contributing to the subsequent separation of thyroid lesions from healthy thyroid tissue.
| Metabolite |
% difference
|
% RSD
|
|||||
|---|---|---|---|---|---|---|---|
| NN vs. H | FA vs. H | TC vs. H | H | NN | FA | TC | |
| Isoleucine | 4.3 | 76.7a,b | 73.8a,b | 27.6 | 19.7 | 96.8 | 34.9 |
| Valine | -1.9 | 83.8a,b | 104.8a,b | 31.0 | 14.8 | 90.2 | 35.6 |
| 3-Hydroxybutyrate | -20.4a,b | -32.2a,b | 5.9 | 31.5 | 27.9 | 55.5 | 86.5 |
| Alanine | 19.1a,b | 81.7a,b | 126.6a,b | 21.9 | 23.6 | 56.4 | 28.7 |
| Acetate | 23.0a | 16.1 | 27.9a,b | 26.5 | 28.6 | 26.8 | 21.9 |
| NAC | -7.7b | -38.6a,b | -34.3a,b | 16.7 | 33.4 | 17.8 | 19.9 |
| Methionine | 32.4a,b | 37.6a,b | 82.5a,b | 24.4 | 24.4 | 25.4 | 23.0 |
| Acetone | -30.2a,b | -36.5a,b | -39.2a,b | 38.3 | 74.2 | 53.5 | 30.6 |
| Glutamate | 53.4a,b | 57.6a,b | 103.9a,b | 29.1 | 54.5 | 43.3 | 40.4 |
| Succinate | 39.7a,b | 71.5 | 63.0a,b | 33.5 | 42.4 | 68.4 | 37.4 |
| Citrate | -24.8 | -66.5a,b | -85.4a,b | 48.1 | 41.7 | 83.5 | 38.0 |
| Creatine | 2.4 | 24.8 | 39.2a,b | 25.8 | 17.7 | 38.2 | 26.0 |
| Choline | 63.4a,b | 13.8 | 72.2a,b | 32.6 | 46.6 | 29.3 | 60.8 |
| PC | 35.0a,b | 25.8 | 30.4 | 33.5 | 25.2 | 54.6 | 55.2 |
| Scyllo-inositol | 48.1 | -3.3 | -63.7a,b | 84.8 | 85.7 | 54.8 | 84.1 |
| Taurine | 14.4b | 24.6 | 44.7a,b | 33.9 | 16.3 | 55.8 | 25.8 |
| Glycine | 33.4a,b | 60.9a,b | 81.2a,b | 25.1 | 32.3 | 44.5 | 33.1 |
| Myo-inositol | 10.7 | -10.8 | -49.1 a,b | 28.3 | 21.7 | 46.8 | 55.9 |
| Lactate | 71.9a,b | 100.2a,b | 187.8a,b | 51.5 | 18.9 | 34.9 | 50.1 |
| β-glucose | 76.0a,b | 162.0a,b | 48.0 | 103.8 | 47.4 | 59.8 | 44.1 |
| Histidine | 34.7a,b | 11.4 | 81.4a,b | 41.8 | 32.8 | 41.5 | 38.6 |
| Tyrosine | 31.5a,b | 75.7a,b | 87.8a,b | 39.8 | 29.2 | 62.8 | 32.4 |
| Phenylalanine | 24.8a,b | 63.6a,b | 99.1a,b | 39.1 | 31.3 | 70.4 | 36.4 |
| Uracil | 35.8 | 58.2 | 72.3a,b | 77.9 | 62.7 | 82.1 | 58.0 |
| Hypoxanthine | 41.1a,b | 47.5a,b | 49.4a,b | 58.7 | 22.5 | 39.8 | 39.4 |
| Formate | 48.9a,b | -1.3 | 21.9 | 78.3 | 50.5 | 58.9 | 88.0 |
Percent difference was calculated from the mean values of the relative signal integrals in each group. Positive values indicate a relatively higher metabolite concentration present in the pathological tissue (NN, FA and TC) compared to the healthy control. RSD: Relative Standard Deviation; NAC: N-acetylated compounds; PC: phosphocholine.
a P<0.05 using Student's t test
b
P<0.05 using the Mann-Whitney-Wilcoxon test