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. 2013 Dec 23;8(12):e83906. doi: 10.1371/journal.pone.0083906

Figure 6. Trajectory analysis paradigm for RPC migration in microfluidic gradients at increasing distances from the EGF source.

Figure 6

Positional tracking of RPCs migrating in steady state gradients of EGF generated in a 13mm bridged μ-lane device over a 24hr period was visualized using Ibidi Migration software to generate wind-rose plots (A-D). A) Cell tracking results are depicted at increasing distances from the source reservoir: 0-500μm (I), 2000-3500μm (II), 5000-7000μm (III). B-D) Depiction of RPC trajectory plot mapping in steady-state gradients of 20, 40, and 400ng/ml EGF analyzed from source reservoirs to sink reservoirs. A trend of reduced chemokinetic migratory dynamics can be observed with increasing EGF concentrations up to 400ng/ml. Red and black traces indicate RPCs with negative and positive COM, respectively.Statistics resulting from trajectory data depicted here analyzed for maximum distance and center of mass are reported below. The x- and y-axis denote RPC displacement in horizontal and vertical directions, respectively.