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. 2013 Dec 23;8(12):e83139. doi: 10.1371/journal.pone.0083139

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© 2013 Dilek et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Proliferation of CD4⁺CD25⁻ T cells stimulated with irradiated allogeneic PBMC at day 5 in presence or not of autologous natural CD4⁺CD25^HighCD127^Low regulatory T cells (nTreg) or clone Treg#1 at a 1∶1 ratio. **P<0.01. (B) The phenotype of Treg#1 clone is compared to effector T cell (Teff) by Flow Cytometry. Control: filled gray, Teff: black line and Treg#1: red line. (C) Costimulatory molecule expression analysis on CD20⁺ B-EBV lymphocytes (pool of 3 cell lines). Control: filled gray and B-EBV cells: green line.