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. 2013 Dec 23;8(12):e83139. doi: 10.1371/journal.pone.0083139

Figure 3. Live-cell dynamic analysis of human Teff and Treg cells in presence of human B-EBV APCs.

Figure 3

(A, C) Screenshots from movies representing Teff or Treg cells stained in green (non-activated cells) or red (activated cells) by Fura2-AM calcium probe in the presence of unstained B-EBV lymphocytes (APCs) in poly-L-lysine coated Labtech. Teff and Treg were pre-incubated with anti-CD28 (FR104) or anti-CD28+ anti-CTLA-4 or nothing (control). Images were analyzed over a period of 20 min. (B, D) Teff and Treg cell motility over 20 minutes period in indicated conditions. (E) Mean of contact times between Teff cells and APCs in the presence or not of various indicated antibodies. (F) Mean of traveled distances by Teff over 20 minutes in the same conditions as above. (G) Mean of Teff cell velocity over 20 minutes in the same conditions as above. (H) Mean of contact times between Treg cells and APCs in the presence or not of various indicated antibodies. (I) Mean of traveled distances by Treg cells over 20 minutes in the same conditions as above. (J) Measurement of Teff cell velocity over 20 minute period in the same conditions as above. All indicated antibodies were used at 10 µg/ml. (n≥30 cells for each condition) ***P<0.001; **P<0.01 and *P<0.05.