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. 2013 Dec 23;8(12):e83384. doi: 10.1371/journal.pone.0083384

Figure 4. Effect of mafosfamide on the Th suppression activity of Treg.

Figure 4

A, Th were co-incubated with the same amount of mafosfamide-pretreated Treg (1∶1), stimulated with antiCD3 antibody and allogenic high-dose irradiated PBMC, and thymidine incorporation was measured in the cell fraction. Treg were not treated or pre-treated with 0.5 and 1.5 µg/ml mafosfamide 24 h before co-cultivation with Th. Co-cultivation and stimulation occurred for 72 h, and thymidine was added to the medium 16 h before cell harvest. The thymidine incorporation in Treg was taken into account by subtracting the thymidine incorporation (cpm/cell) in mono-cultivated and stimulated Treg from the total population. Data were corrected for cytotoxicity i.e. the dead cell Treg fraction was not taken into account. Data are the mean of three independent experiments. B, Relative suppression activity of Treg. Data are from Fig. 4B. * p<0.05. We should note that in this assay the data were corrected as to cytotoxicity.