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. 2013 Dec 23;8(12):e83384. doi: 10.1371/journal.pone.0083384

Figure 5. Helios expression in viable Treg.

Figure 5

A, Dot plots of control and mafosfamide (2 µg/ml, 48 h) treated Treg. The living fraction was gated (P1) and Helios+ cells were marked green in this fraction (designed as P2). Left panel shows representative graphs of untreated Treg; right panel of treated Treg. B, Quantification of Helios expressing Treg, Th and CTL determined 48 h after treatment with 2 µg/ml mafosfamide. C, Quantification of data with the non-treated control set to 1. * p<0.05 significance.