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. 2013 Sep 26;25(1):81–91. doi: 10.1681/ASN.2012050486

Figure 2.

Figure 2.

Accentuation of canonical TGF-β signaling in Pkd1KO/+; Fbn1C1039G/+ double heterozygous aortas. (A–D) Aortic sections from 6-month-old mice stained with antisera to pSmad2, a marker of canonical TGF-β signaling. Increased nuclear pSmad2 is observed in the Fbn1C1039G/+mice (C) relative to wild-type mice (A) and Pkd1KO/+(B). (D) Staining for pSmad2 is further increased in Pkd1KO/+;Fbn1C1039G/+ aortas. (E) Blinded quantitative histologic evaluation of phosphorylated Smad2 expression (±SEM) (n=5). (F–I) Aortic sections from 6-month-old mice stained with antisera to CTGF, a prototypical TGF-β response gene. There is increased CTGF expression in Pkd1KO/+; Fbn1C1039G/+ mice (I) compared with all of the other genotypes (F–H). (J) Blinded quantitative histologic evaluation of CTGF expression (±SEM) (n=5). *P<0.05; **P<0.01. Scale bars, 25 µm.