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. 2013 Sep 5;25(1):33–42. doi: 10.1681/ASN.2012121218

Figure 3.

Figure 3.

Angpt1 repletion ameliorates diabetes-mediated albuminuria and glomerular endothelial cells proliferation. Albuminuria (geometric mean, 95% confidence intervals) was increased in diabetic mice compared with nondiabetic mice (n=10–12/group; *P=0.001) (A); significant amelioration of albuminuria was observed in diabetic mice with restored Angpt1 levels (n=10–12/group; #P=0.003). Representative image on transmission electron microscopy (scale bar: 2 μm) (B) and quantitative electron microscopy analysis of glomerular parameters: mesangial volume fraction (VvMes) (C), glomerular basement (GBM) width (D), podocyte density (E), and foot process width (F) in nondiabetic Pod/+, diabetic Pod/+ and Pod/Angpt1 mice after 10 weeks of doxycycline (DOX) administration. VvMes and GBM width were increased in diabetic compared with nondiabetic mice (n=9–12/group; *P<0.01); Angpt1 repletion did not affect these parameters. Angpt1 repletion prevented the diabetes-induced increase in total glomerular Ki67-positive cells and proliferating endothelia (EC) (G and H, n=4/group; *P<0.01, #P<0.01). Representative image showing a Ki67-positive glomerular endothelial cell (I) (magnification ×60). D, diabetic; ND, nondiabetic. *indicates comparisons between nondiabetic and diabetic states; # indicates the comparisons between diabetic states without and with Angpt1 repletion.